Device

Part:BBa_K2074007:Design

Designed by: Tong Tong   Group: iGEM16_FAFU-CHINA   (2016-10-17)


Cry11Aa+2A+Cyt1 expressed in C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 2336
    Illegal NgoMIV site found at 2684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 704
    Illegal BsaI.rc site found at 899
    Illegal BsaI.rc site found at 2114
    Illegal BsaI.rc site found at 3203
    Illegal SapI.rc site found at 2095


Design Notes

none


Source

HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry11Aa and Cyt1 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.

References